Cre Lox

The crelox system is one of the most powerful and versatile tools developed for mouse genetics.

Cre lox. 4 sauer b henderson n. Inversion deletion and translocation. Have questions about your order deposit or a plasmid. Floxd shrnacreshrnalox stop lox shrnacreshrna1 2shrna imagepng 4gene switch.

Functional expression of the cre lox site specific recombination system in the yeast saccharomyces cerevisiae. The proper insertion of a loxp flanked stop sequence transcriptional termination element. A cre mouse contains a cre recombinase transgene under the direction of a tissue specific promoter. To use cre lox technology an investigator has to produce a cre lox mouse typically by breeding a cre mouse to a loxp mouse.

A loxp mouse contains two loxp sites that flank a genomic segment of interest the floxed locus. Learn about the latest plasmid technologies and research tools. Proc natl acad sci u s a. A lambdaplasmid crelox hybrid vector for large genomic 18kb fragment insertions and fungal genomic library construction.

Annual review of neuroscience 2020 07 15. The schematic below shows the three types of rearrangements. Crelox is usually used to make knockout alleles but it can also be used to activate gene expression. Addgene is a nonprofit plasmid repository.

The cre lox system derived from p1 bacteriophage is a potent and specific system for controlling gene expression. Sign up for our newsletter. The protein cre recombinase recognizes 34 bp loxp sites and the orientation and location of the loxp sites determines how the genetic material will be rearranged. Site specific dna recombination in mammalian cells by the cre recombinase of bacteriophage p1.

It gives mouse researchers sophisticated control over the location and timing of gene expression.